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Anaesthesia

Title:		The effects of sevoflurane and desflurane in vitro on platelet-leukocyte adhesion in whole blood
Author(s):		Horn, N. A.de Rossi, L.Robitzsch, T.Hecker, K. E.Hutschenreuter, G.Rossaint, R.
Address:		Department of Anaesthesiology, Rheinisch-Westfalische Technische Hochschule Aachen, Pauwelsstrasse 30, 52074 Aachen, Germany. nhorn@ukaachen.de
Year:		2003 Apr
Journal Title:		Anaesthesia
Page Number:		312-9
Language:		eng
Volume:		58
Issue:		4
ISSN/ISBN:		0003-2409 (Print). 0003-2409 (Linking)
Abstract:		The interaction between platelets and leukocytes plays an important role in inflammatory and thrombotic processes. We investigated whether the volatile anaesthetics sevoflurane and desflurane alter the formation of platelet-leukocyte aggregates and the expression of P-selectin on platelets. Whole blood was incubated with 1 and 2 minimum alveolar concentration (MAC) sevoflurane or desflurane. Unstimulated and adenosine diphosphate, or thrombin receptor agonist peptide-6-stimulated samples were stained with fluorochrome-conjugated antibodies. The formation of platelet-leukocyte conjugates and the expression of P-selectin on platelets were measured using flow cytometry. Sevoflurane was found to enhance the binding of platelets to lymphocytes, neutrophils and monocytes, it also increased the expression of P-selectin on platelets especially in the stimulated samples. Desflurane decreased the percentage of lymphocyte-platelet, neutrophil-platelet and monocyte-platelet conjugates principally in unstimulated samples. The results show that these two volatile anaesthetics have differing effects on the formation of platelet-leukocyte conjugates in vitro. Sevoflurane also enhanced the expression of P-selectin on platelets.