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Appl Environ Microbiol


Title:Geosmin and Related Volatiles in Bioreactor-Cultured Streptomyces citreus CBS 109.60
Author(s):Pollak, F. C.Berger, R. G.
Address:
Year:1996 Apr
Journal Title:Appl Environ Microbiol
Page Number:1295-1299
Language:ENG
Volume:62
Issue:4
ISSN/ISBN:1098-5336 (Electronic). 0099-2240 (Linking)
Abstract:Streptomyces citreus CBS 109.60 produced geosmin and a complex pattern of other volatile compounds during cultivation in a 2.5-liter laboratory bioreactor. Volatiles were isolated from disrupted cells, from the culture medium, and from the waste air of the bioreactor by adsorption on Lewatit OC 1064MD. Quantitative and qualitative analyses were carried out using capillary gas chromatography and coupled gas chromatography-mass spectroscopy. S. citreus produced 56 volatile compounds, which were mainly terpenoids but also included aliphatic ketones, alcohols, esters, pyrazines, furan(on)es, and aromatic types during the growth phase. The major components were geosmin and a germacradienol. A biosynthetic pathway for geosmin including eudesmanolides is proposed.

 
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